Reply to the letter to the editor 'Journey to a faraway land' by Alkan et al.

نویسندگان

  • A D Hartkopf
  • S Y Brucker
  • F-A Taran
چکیده

supporting its role in CRC metastatic development [1]. However, the precise status of miR-29c in metastatic CRC tissues is a major question to be fully investigated. Thus, we quantified miR-29c in primary and paired metastatic tissues from 17 CRC patients, 12 with liver metastasis and 5 with lung metastasis, using Taqman Low Density Arrays (TLDAs) panel A (Applied Biosystems). A pathologist reviewed the specimens to further confirm the diagnosis. All samples were taken anonymously and the ethical committee and institutional review board approved the project. Relative gene expression analysis was carried out using the 2 method and U6B as internal control. We observed similar miR-29c expression between primary and metastatic tissues from those 12 CRC patients with liver metastasis. However, almost threefold increased miR-29c levels were found in the metastatic tissues of those cases with lung metastasis. We then investigated whether these differences were due to expression changes in the primary tissues or dependent on the metastatic site. Interestingly, miR-29c showed significantly lower expression in primary CRC tissues from patients with lung metastasis compared with those cases with liver metastasis (P = 0.046). No differences were observed when comparing miR-29c between liver and lung CRC metastatic tissues (P = 0.934). In summary, our results would indicate that deeper miR-29c downregulation would be required in the premetastatic CRC cell to develop lung metastasis than liver metastasis. In addition, the potential predictive value of miR-29c determining the CRC metastatic niche should be further confirmed in forthcoming studies.

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عنوان ژورنال:
  • Annals of oncology : official journal of the European Society for Medical Oncology

دوره 26 10  شماره 

صفحات  -

تاریخ انتشار 2015